These
studies use imaging techniques to assess the effects of ethanol (R21
AA022168) and toluene (DA01351) on cortical neuron excitability.
Approaches include in vivo 2-photon microscopy (in collaboration with
MUSC colleagues Dr. Prakash Kara and. Dr. Andy Shih), in vivo fiber
photometry, viral-mediated delivery of genetically encoded calcium and
glutamate sensors and use of GCaMP6 expressing transgenic mice.
2P in vivo calcium Imaging of mouse visual cortex-acute ethanol reduces sensory evoked calcium transients
2P in vivo glutamate imaging of mouse visual cortex-acute ethanol reduces sensory evoked changes in glutamate
Effects
of chronic intermittent ethanol (CIE) exposure on mouse visual cortex.
CIE reduces orientation selectivity while increasing peak amplitude of
responses.
In vivo fiber photometry of
mouse prefrontal cortex. Traces show neuronal calcium transients from
GCaMP6 mouse before, during and after exposure to toluene vapor.